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By the time Sanger sequencing was commercialized, the groundwork for an entirely new sequencing chemistry was already well underway. In 1985, Swedish biochemists Pål Nyren and Arne Lundin published a paper illustrating a procedure that measured the concentration of a molecule, called pyrophosphate (PPi), using an enzymatic cascade that emits light. In early 1986, Nyren realized that the method he’d helped develop could be applied to DNA sequencing, because PPi is naturally produced as a byproduct of DNA synthesis.5
A major utility company has admitted supplying water unfit for human consumption after a parasite outbreak in Devon made almost 150 people sick.，详情可参考快连下载-Letsvpn下载
Фото: Александр Миридонов / Коммерсантъ